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rabbit anti- gnat1  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology rabbit anti- gnat1
    Rabbit Anti Gnat1, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+gnat1/gnat1+antibody/pm39531499-292-6-12
    Average 90 stars, based on 1 article reviews
    rabbit anti- gnat1 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Retinal Layer Separation (ReLayS) method enables the molecular analysis of photoreceptor segments and cell bodies, as well as the inner retina
    Article Snippet: Briefly, 5 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upmu$$\end{document} μ g of proteins were run on a 10% SDS PAGE and blotted onto a nitrocellulose membrane (BioRad, 1620112) using the Trans-Blot Turbo Transfer System (BioRad, 1704150). .. The membrane was blocked in 5 % nonfat dry milk (Bio-Rad Laboratories; Blotting-Grade Blocker 1706404) in TBST (Tris-Buffered Saline with 0.1% Tween) for 1 h at RT and incubated with the following primary antibodies overnight at 4 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^{\circ }$$\end{document} ∘ C: rabbit anti-PDE6 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upalpha$$\end{document} α (1:750; Abcam, ab5659), rabbit anti-KHSRP (1:5000; Novus Biologicals, NBP1-18910), rabbit anti-PKC \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upalpha$$\end{document} α (1:1000; Sigma-Aldrich, P4334), rabbit anti-PKM2 (1:1000; Cell Signaling, 3198S), rabbit anti-CS (1:1000; GeneTex, GTX110624), mouse anti-SYP (1:1000; Novocastra, NCL-L-SYNAP-299), rabbit anti-SAG (1:500; Affinity BioReagents, PA1-731), mouse anti-BRN3A (1:500; Chemicon, MAB1585), rabbit anti-GNAT1 (1:200; Santa Cruz Biotechnology, sc-389), mouse anti-RHO (1:8000; Sigma-Aldrich, O4886), rabbit anti-STAT3 (1:500; Cell Signaling, D3Z2G), and mouse anti-GFAP (1:1000; Sigma-Aldrich, G3893). ..

    Saline:

    Article Title: Retinal Layer Separation (ReLayS) method enables the molecular analysis of photoreceptor segments and cell bodies, as well as the inner retina
    Article Snippet: Briefly, 5 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upmu$$\end{document} μ g of proteins were run on a 10% SDS PAGE and blotted onto a nitrocellulose membrane (BioRad, 1620112) using the Trans-Blot Turbo Transfer System (BioRad, 1704150). .. The membrane was blocked in 5 % nonfat dry milk (Bio-Rad Laboratories; Blotting-Grade Blocker 1706404) in TBST (Tris-Buffered Saline with 0.1% Tween) for 1 h at RT and incubated with the following primary antibodies overnight at 4 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^{\circ }$$\end{document} ∘ C: rabbit anti-PDE6 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upalpha$$\end{document} α (1:750; Abcam, ab5659), rabbit anti-KHSRP (1:5000; Novus Biologicals, NBP1-18910), rabbit anti-PKC \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upalpha$$\end{document} α (1:1000; Sigma-Aldrich, P4334), rabbit anti-PKM2 (1:1000; Cell Signaling, 3198S), rabbit anti-CS (1:1000; GeneTex, GTX110624), mouse anti-SYP (1:1000; Novocastra, NCL-L-SYNAP-299), rabbit anti-SAG (1:500; Affinity BioReagents, PA1-731), mouse anti-BRN3A (1:500; Chemicon, MAB1585), rabbit anti-GNAT1 (1:200; Santa Cruz Biotechnology, sc-389), mouse anti-RHO (1:8000; Sigma-Aldrich, O4886), rabbit anti-STAT3 (1:500; Cell Signaling, D3Z2G), and mouse anti-GFAP (1:1000; Sigma-Aldrich, G3893). ..

    Incubation:

    Article Title: Retinal Layer Separation (ReLayS) method enables the molecular analysis of photoreceptor segments and cell bodies, as well as the inner retina
    Article Snippet: Briefly, 5 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upmu$$\end{document} μ g of proteins were run on a 10% SDS PAGE and blotted onto a nitrocellulose membrane (BioRad, 1620112) using the Trans-Blot Turbo Transfer System (BioRad, 1704150). .. The membrane was blocked in 5 % nonfat dry milk (Bio-Rad Laboratories; Blotting-Grade Blocker 1706404) in TBST (Tris-Buffered Saline with 0.1% Tween) for 1 h at RT and incubated with the following primary antibodies overnight at 4 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$^{\circ }$$\end{document} ∘ C: rabbit anti-PDE6 \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upalpha$$\end{document} α (1:750; Abcam, ab5659), rabbit anti-KHSRP (1:5000; Novus Biologicals, NBP1-18910), rabbit anti-PKC \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$$\upalpha$$\end{document} α (1:1000; Sigma-Aldrich, P4334), rabbit anti-PKM2 (1:1000; Cell Signaling, 3198S), rabbit anti-CS (1:1000; GeneTex, GTX110624), mouse anti-SYP (1:1000; Novocastra, NCL-L-SYNAP-299), rabbit anti-SAG (1:500; Affinity BioReagents, PA1-731), mouse anti-BRN3A (1:500; Chemicon, MAB1585), rabbit anti-GNAT1 (1:200; Santa Cruz Biotechnology, sc-389), mouse anti-RHO (1:8000; Sigma-Aldrich, O4886), rabbit anti-STAT3 (1:500; Cell Signaling, D3Z2G), and mouse anti-GFAP (1:1000; Sigma-Aldrich, G3893). ..

    Immunofluorescence:

    Article Title: Drug-inducible synergistic gene silencing with multiple small hairpin RNA molecules for gene function study in animal model
    Article Snippet: .. The antibodies and working dilutions used for immunofluorescence analysis and immunoblotting were as follows: rabbit anti-SIRT1 (1:500–2,000; #1104-1, Epitomics), anti-rhodopsin (1:500–2,000; #sc-57433, Santa Cruz), rabbit anti-GNAT1 (1:200–1,000; # sc-389, Santa Cruz), goat anti-CNGA1 (1:500–1,000; #sc-13694, Santa Cruz), goat anti-PDC (1:500–1,000; #sc-18413, Santa Cruz), anti-PDE6b (1:200–1,000; #sc-30717, Santa Cruz), anti-actin (1:2,000–10,000; #A5228, Sigma-Aldrich). .. Corresponding IgG antibodies conjugated with Alexa Fluor ® dye (594 or 488; Invitrogen) were used as secondary antibodies for the immunofluorescence analysis.

    Western Blot:

    Article Title: Drug-inducible synergistic gene silencing with multiple small hairpin RNA molecules for gene function study in animal model
    Article Snippet: .. The antibodies and working dilutions used for immunofluorescence analysis and immunoblotting were as follows: rabbit anti-SIRT1 (1:500–2,000; #1104-1, Epitomics), anti-rhodopsin (1:500–2,000; #sc-57433, Santa Cruz), rabbit anti-GNAT1 (1:200–1,000; # sc-389, Santa Cruz), goat anti-CNGA1 (1:500–1,000; #sc-13694, Santa Cruz), goat anti-PDC (1:500–1,000; #sc-18413, Santa Cruz), anti-PDE6b (1:200–1,000; #sc-30717, Santa Cruz), anti-actin (1:2,000–10,000; #A5228, Sigma-Aldrich). .. Corresponding IgG antibodies conjugated with Alexa Fluor ® dye (594 or 488; Invitrogen) were used as secondary antibodies for the immunofluorescence analysis.



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    Proteintech rabbit α mouse gnat1 igg
    Confirmation of DKO phenotype and genotype. (A) Representative immunofluorescence images of retinal cross-sections stained for rod α-transducin (top, <t>Gnat1,</t> gold) and cone α-transducin (bottom, Gnat2, gold) in a control mouse (left) and a DKO mouse (right). White dashed lines delineate the photoreceptor outer segments layer. For Gnat1 staining (top) red arrows point to the presence of robust Gnat1 staining in the outer segments of rod photoreceptors. This staining is undetectable in DKO mice (top, right, red arrow), suggesting a successful knockout of Gnat1 expression. In both images, a large amount of autofluorescence is present at the border between the photoreceptor layer and outer nuclear layer, which was attributed to non-specific staining by the secondary antibody (as shown by a no-primary antibody control, data not shown). For Gnat2 staining (bottom), cone α-transducin expression was clearly present in the oval-shaped outer segments of cone photoreceptors (indicated by red arrows) in the control animals (bottom, left). This staining pattern was totally absent from our DKO retinal slices (bottom, right), suggesting that Gnat2 expression was significantly reduced. (B) Example 1% agarose gels showing the genotyping of a control mouse (left) and a DKO mouse (right), as described in our methods. For gnat1, the control and knockout bands were located at ∼300 and ∼200 bp, respectively. For Gnat2, the control and knockout bands were located at ∼480 and ∼300 bp, respectively. Our DKO animals showed the proper homozygous band pattern we would expect.
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    Santa Cruz Biotechnology rabbit anti gnat1
    Confirmation of DKO phenotype and genotype. (A) Representative immunofluorescence images of retinal cross-sections stained for rod α-transducin (top, <t>Gnat1,</t> gold) and cone α-transducin (bottom, Gnat2, gold) in a control mouse (left) and a DKO mouse (right). White dashed lines delineate the photoreceptor outer segments layer. For Gnat1 staining (top) red arrows point to the presence of robust Gnat1 staining in the outer segments of rod photoreceptors. This staining is undetectable in DKO mice (top, right, red arrow), suggesting a successful knockout of Gnat1 expression. In both images, a large amount of autofluorescence is present at the border between the photoreceptor layer and outer nuclear layer, which was attributed to non-specific staining by the secondary antibody (as shown by a no-primary antibody control, data not shown). For Gnat2 staining (bottom), cone α-transducin expression was clearly present in the oval-shaped outer segments of cone photoreceptors (indicated by red arrows) in the control animals (bottom, left). This staining pattern was totally absent from our DKO retinal slices (bottom, right), suggesting that Gnat2 expression was significantly reduced. (B) Example 1% agarose gels showing the genotyping of a control mouse (left) and a DKO mouse (right), as described in our methods. For gnat1, the control and knockout bands were located at ∼300 and ∼200 bp, respectively. For Gnat2, the control and knockout bands were located at ∼480 and ∼300 bp, respectively. Our DKO animals showed the proper homozygous band pattern we would expect.
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    Image Search Results


    Confirmation of DKO phenotype and genotype. (A) Representative immunofluorescence images of retinal cross-sections stained for rod α-transducin (top, Gnat1, gold) and cone α-transducin (bottom, Gnat2, gold) in a control mouse (left) and a DKO mouse (right). White dashed lines delineate the photoreceptor outer segments layer. For Gnat1 staining (top) red arrows point to the presence of robust Gnat1 staining in the outer segments of rod photoreceptors. This staining is undetectable in DKO mice (top, right, red arrow), suggesting a successful knockout of Gnat1 expression. In both images, a large amount of autofluorescence is present at the border between the photoreceptor layer and outer nuclear layer, which was attributed to non-specific staining by the secondary antibody (as shown by a no-primary antibody control, data not shown). For Gnat2 staining (bottom), cone α-transducin expression was clearly present in the oval-shaped outer segments of cone photoreceptors (indicated by red arrows) in the control animals (bottom, left). This staining pattern was totally absent from our DKO retinal slices (bottom, right), suggesting that Gnat2 expression was significantly reduced. (B) Example 1% agarose gels showing the genotyping of a control mouse (left) and a DKO mouse (right), as described in our methods. For gnat1, the control and knockout bands were located at ∼300 and ∼200 bp, respectively. For Gnat2, the control and knockout bands were located at ∼480 and ∼300 bp, respectively. Our DKO animals showed the proper homozygous band pattern we would expect.

    Journal: Frontiers in Cellular Neuroscience

    Article Title: Robust visual cortex evoked potentials (VEP) in Gnat1 and Gnat2 knockout mice

    doi: 10.3389/fncel.2022.1090037

    Figure Lengend Snippet: Confirmation of DKO phenotype and genotype. (A) Representative immunofluorescence images of retinal cross-sections stained for rod α-transducin (top, Gnat1, gold) and cone α-transducin (bottom, Gnat2, gold) in a control mouse (left) and a DKO mouse (right). White dashed lines delineate the photoreceptor outer segments layer. For Gnat1 staining (top) red arrows point to the presence of robust Gnat1 staining in the outer segments of rod photoreceptors. This staining is undetectable in DKO mice (top, right, red arrow), suggesting a successful knockout of Gnat1 expression. In both images, a large amount of autofluorescence is present at the border between the photoreceptor layer and outer nuclear layer, which was attributed to non-specific staining by the secondary antibody (as shown by a no-primary antibody control, data not shown). For Gnat2 staining (bottom), cone α-transducin expression was clearly present in the oval-shaped outer segments of cone photoreceptors (indicated by red arrows) in the control animals (bottom, left). This staining pattern was totally absent from our DKO retinal slices (bottom, right), suggesting that Gnat2 expression was significantly reduced. (B) Example 1% agarose gels showing the genotyping of a control mouse (left) and a DKO mouse (right), as described in our methods. For gnat1, the control and knockout bands were located at ∼300 and ∼200 bp, respectively. For Gnat2, the control and knockout bands were located at ∼480 and ∼300 bp, respectively. Our DKO animals showed the proper homozygous band pattern we would expect.

    Article Snippet: Primary antibodies used were Rabbit α mouse Gnat1 IgG (Ref: 55167-1-AP, Proteintech, Rosemont, IL, USA) or Rabbit α mouse GNAT2 IgG (Ref: PA5-22340, Thermo Fisher Scientific), both diluted to 1:250.

    Techniques: Immunofluorescence, Staining, Control, Knock-Out, Expressing